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directpcr lysis reagent 102-t  (ViaGen Inc)

 
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    ViaGen Inc directpcr lysis reagent 102-t
    Directpcr Lysis Reagent 102 T, supplied by ViaGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/directpcr+reagents/directpcr+lysis+reagent/pmc09481912__mmc1-163-5-8
    Average 90 stars, based on 1 article reviews
    directpcr lysis reagent 102-t - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: WNK1 Protein Kinase Regulates Embryonic Cardiovascular Development through the OSR1 Signaling Cascade
    Article Snippet: For genotyping, mouse tail tips, ear punches, toe clips, or portions of yolk sacs of embryos were digested overnight in Viagen DirectPCR reagents with 0.2 mg/ml proteinase K at 55 °C; heat-inactivated at 85 °C for 45 min; and analyzed by PCRs as described (14).

    Article Title: WNK4 kinase is a physiological intracellular chloride sensor
    Article Snippet: For genotyping, mice tail clips were digested overnight in Viagen DirectPCR reagents with 0.2 mg/dL proteinase K at 56 °C and heat-inactivated by boiling in water bath for 5 min or at 85 °C for 45 min. Genomic DNA obtained from mice tail was used for genotyping PCR with the setting of hot start at 95 °C for 5 min, followed with 40 cycles at 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 30 s, and ended with the extension step of 72 °C for 7 min.

    Article Title: Differential roles of WNK4 in regulation of NCC in vivo
    Article Snippet: For genotyping, mice tail clips were digested overnight in Viagen DirectPCR reagents with 0.2 mg/ml proteinase K at 55°C, heat-inactivated by boiling in water bath for 5 min or at 85°C for 45 min. Genomic DNA was analyzed by using PCR using primers as described below.

    Polymerase Chain Reaction:

    Article Title: Endothelial-Specific Expression of WNK1 Kinase Is Essential for Angiogenesis and Heart Development in Mice
    Article Snippet: .. Mouse tail tips, ear punches, toe clips, or portions of yolk sacs of embryos were digested in Viagen DirectPCR reagents (Viagen Biotech, Los Angeles, CA) with 0.2 mg/ml proteinase K at 55°C overnight, heat-inactivated at 85°C for 45 minutes, and analyzed by polymerase chain reaction (PCR). ..

    Article Title: Astrocytic expression of Parkinson's disease-related A53T α-synuclein causes neurodegeneration in mice
    Article Snippet: .. Genotypes were determined by PCR analysis of tail DNA extracted by DirectPCR Reagents (VIAGEN Biotech, CA). ..



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